Proliferative growth of SARS coronavirus in Vero E6 cells.
Identifieur interne : 005E98 ( Main/Exploration ); précédent : 005E97; suivant : 005E99Proliferative growth of SARS coronavirus in Vero E6 cells.
Auteurs : M-L Ng [Singapour] ; S-H Tan [Singapour] ; E-E See [Singapour] ; E-E Ooi [Singapour] ; A-E Ling [Singapour]Source :
- The Journal of general virology [ 0022-1317 ] ; 2003.
Descripteurs français
- KwdFr :
- Animaux, Appareil de Golgi (ultrastructure), Appareil de Golgi (virologie), Cellules Vero, Cytoplasme (ultrastructure), Cytoplasme (virologie), Facteurs temps, Humains, Membrane cellulaire (ultrastructure), Membrane cellulaire (virologie), Microscopie électronique, Réplication virale, Spécificité d'espèce, Virus du SRAS (croissance et développement), Virus du SRAS (isolement et purification), Virus du SRAS (ultrastructure).
- MESH :
- croissance et développement : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Appareil de Golgi, Cytoplasme, Membrane cellulaire.
- ultrastructure : Animaux, Appareil de Golgi, Cellules Vero, Cytoplasme, Facteurs temps, Humains, Membrane cellulaire, Microscopie électronique, Réplication virale, Spécificité d'espèce, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Cell Membrane (ultrastructure), Cell Membrane (virology), Chlorocebus aethiops, Cytoplasm (ultrastructure), Cytoplasm (virology), Golgi Apparatus (ultrastructure), Golgi Apparatus (virology), Humans, Microscopy, Electron, SARS Virus (growth & development), SARS Virus (isolation & purification), SARS Virus (ultrastructure), Species Specificity, Time Factors, Vero Cells, Virus Replication.
- MESH :
- growth & development : SARS Virus.
- isolation & purification : SARS Virus.
- ultrastructure : Cell Membrane, Cytoplasm, Golgi Apparatus, SARS Virus.
- virology : Cell Membrane, Cytoplasm, Golgi Apparatus.
- Animals, Chlorocebus aethiops, Humans, Microscopy, Electron, Species Specificity, Time Factors, Vero Cells, Virus Replication.
Abstract
An isolate of SARS coronavirus (strain 2003VA2774) was obtained from a patient and used to infect Vero E6 cells. The replication cycle of the virus was followed from 1 to 30 h post-infection (p.i.). It was surprising to observe the swift growth of this human virus in Vero cells. Within the first hour of infection, the most obvious ultrastructural change was the proliferation of the Golgi complexes and related vesicles accompanied by swelling of some of the trans-Golgi sacs. Extracellular virus particles were present by 5 h p.i. in about 5 % of the cells and this increased dramatically to about 30 % of the cell population within an hour (6 h p.i.). Swollen Golgi sacs contained virus nucleocapsids at different stages of maturation. These virus precursors were also in large vacuoles and in close association with membrane whorls. The membrane whorls could be the replication complexes, since they appeared rather early in the replication cycle. As infection progressed from 12 to 21 h p.i., the cytoplasm of the infected cells was filled with numerous large, smooth-membraned vacuoles containing a mixture of mature virus and spherical cores. Several of these vacuoles were close to the cell periphery, ready to export out the mature progeny virus particles via exocytosis. By 24 to 30 h p.i., crystalline arrays of the extracellular virus particles were seen commonly at the cell surface.
DOI: 10.1099/vir.0.19505-0
PubMed: 14645910
Affiliations:
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L" uniqKey="Ng M" first="M-L" last="Ng">M-L Ng</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, 5 Science Drive 2, National University of Singapore, 117597, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, 5 Science Drive 2, National University of Singapore, 117597</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Tan, S H" sort="Tan, S H" uniqKey="Tan S" first="S-H" last="Tan">S-H Tan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, 5 Science Drive 2, National University of Singapore, 117597, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, 5 Science Drive 2, National University of Singapore, 117597</wicri:regionArea><orgName type="university">Université nationale de 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(virology)</term><term>Chlorocebus aethiops</term><term>Cytoplasm (ultrastructure)</term><term>Cytoplasm (virology)</term><term>Golgi Apparatus (ultrastructure)</term><term>Golgi Apparatus (virology)</term><term>Humans</term><term>Microscopy, Electron</term><term>SARS Virus (growth & development)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (ultrastructure)</term><term>Species Specificity</term><term>Time Factors</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Appareil de Golgi (ultrastructure)</term><term>Appareil de Golgi (virologie)</term><term>Cellules Vero</term><term>Cytoplasme (ultrastructure)</term><term>Cytoplasme (virologie)</term><term>Facteurs temps</term><term>Humains</term><term>Membrane cellulaire (ultrastructure)</term><term>Membrane cellulaire (virologie)</term><term>Microscopie électronique</term><term>Réplication virale</term><term>Spécificité d'espèce</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Membrane</term><term>Cytoplasm</term><term>Golgi Apparatus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Appareil de Golgi</term><term>Cytoplasme</term><term>Membrane cellulaire</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Membrane</term><term>Cytoplasm</term><term>Golgi Apparatus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Microscopy, Electron</term><term>Species Specificity</term><term>Time Factors</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Appareil de Golgi</term><term>Cellules Vero</term><term>Cytoplasme</term><term>Facteurs temps</term><term>Humains</term><term>Membrane cellulaire</term><term>Microscopie électronique</term><term>Réplication virale</term><term>Spécificité d'espèce</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An isolate of SARS coronavirus (strain 2003VA2774) was obtained from a patient and used to infect Vero E6 cells. The replication cycle of the virus was followed from 1 to 30 h post-infection (p.i.). It was surprising to observe the swift growth of this human virus in Vero cells. Within the first hour of infection, the most obvious ultrastructural change was the proliferation of the Golgi complexes and related vesicles accompanied by swelling of some of the trans-Golgi sacs. Extracellular virus particles were present by 5 h p.i. in about 5 % of the cells and this increased dramatically to about 30 % of the cell population within an hour (6 h p.i.). Swollen Golgi sacs contained virus nucleocapsids at different stages of maturation. These virus precursors were also in large vacuoles and in close association with membrane whorls. The membrane whorls could be the replication complexes, since they appeared rather early in the replication cycle. As infection progressed from 12 to 21 h p.i., the cytoplasm of the infected cells was filled with numerous large, smooth-membraned vacuoles containing a mixture of mature virus and spherical cores. Several of these vacuoles were close to the cell periphery, ready to export out the mature progeny virus particles via exocytosis. By 24 to 30 h p.i., crystalline arrays of the extracellular virus particles were seen commonly at the cell surface.</div></front></TEI><affiliations><list><country><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Ng, M L" sort="Ng, M L" uniqKey="Ng M" first="M-L" last="Ng">M-L Ng</name></noRegion><name sortKey="Ling, A E" sort="Ling, A E" uniqKey="Ling A" first="A-E" last="Ling">A-E Ling</name><name sortKey="Ooi, E E" sort="Ooi, E E" uniqKey="Ooi E" first="E-E" last="Ooi">E-E Ooi</name><name sortKey="See, E E" sort="See, E E" uniqKey="See E" first="E-E" last="See">E-E See</name><name sortKey="Tan, S H" sort="Tan, S H" uniqKey="Tan S" first="S-H" last="Tan">S-H Tan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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